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Analyse af RNA Processing Reaktioner Brug Cell Free Systems: 3 'End spaltning af præ-mRNA Underlag

Article DOI: 10.3791/51309-v • 09:16 min • May 3rd, 2014
May 3rd, 2014 •
1, 1, 2, 1
1Department of Infectious Diseases, The Scripps Research Institute, 2Department of Chemistry, City College of New York

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RNA-polymerase II syntetiserer en precursor RNA, som strækker sig ud over 3'-enden af ​​det modne mRNA. Den ende af det modne RNA genereres cotransskriptionelt på et sted dikteret af RNA-sekvenser via endonucleaseaktivitet af spaltning kompleks. Her har vi detaljeret metode til at studere spaltningsreaktioner in vitro.

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Infektionssygdomme Spaltning polyadenylering mRNA-processering Kerneekstrakter 3 'Behandling Complex
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