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Journal / Neuroscience / Neonatal pial Surface Elektroporation
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JoVE Journal Neuroscience
Neonatal pial Surface Elektroporation
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Neonatal pial Surface Elektroporation

Article DOI: 10.3791/51319-v • 06:22 min • May 7th, 2014
May 7th, 2014 •
1, 2, *1, *2
1Department of Neurosurgery, Regenerative Medicine Institute, Cedars-Sinai Medical Center, 2Department of Biomedical Sciences, Regenerative Medicine Institute, Cedars-Sinai Medical Center
* These authors contributed equally

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Pial overflade er en unik stamfader zone i CNS, som modtager stigende opmærksomhed. Heri vi detaljeret en fremgangsmåde til hurtig genetisk manipulation af denne progenitor zone anvendelse af en modificeret elektroporation metode. Denne procedure kan bruges til cellulære og molekylære undersøgelser af cellelinier og signalveje, der er involveret i celle differentiering og at belyse skæbne og egenskaber datterceller.

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Neuroscience Developmental Biology neonatal gnaver skæbne kortlægning afstamning opsporing genetisk manipulation plasmid-DNA piggyBac tol2 transposon TCHD elektroporation
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