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Journal / Biology / Imaging Plasma Membran Deformasjoner Med pTIRFM
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Biology

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Imaging Plasma Membran Deformasjoner Med pTIRFM

Article DOI: 10.3791/51334-v • 12:28 min • April 2nd, 2014
April 2nd, 2014 •
1, 1, 1, 1
1Department of Biological Sciences, Wayne State University

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Polarisering-baserte Total Internal Reflection fluorescens mikroskopi (pTIRFM) muliggjør sanntidsdeteksjon av dynamikk cellemembranen. Denne artikkelen beskriver gjennomføringen av pTIRFM for studiet av membran ombygging under regulert eksocytose. Teknikken er generalizable til andre prosesser i cellebiologi som direkte eller indirekte involverer endringer i membranen form.

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Biokjemi kromaffinceller lipid bilayers Mikroskopi fluorescens Polarisering eksocytose membran TIRF pTIRF chromaffin polarisering vesikkel
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