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Journal / Neuroscience / 共识脑源性蛋白，人类和小鼠脑蛋白质组使用两个2D-DIGE和Mini双向电泳免疫印迹法研究提取协议…
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Neuroscience

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JoVE Journal Neuroscience
共识脑源性蛋白，人类和小鼠脑蛋白质组使用两个2D-DIGE和Mini双向电泳免疫印迹法研究提取协议
Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum
Journal (Neuroscience)
Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum
Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
Journal (Neuroscience)
Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
 
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共识脑源性蛋白，人类和小鼠脑蛋白质组使用两个2D-DIGE和Mini双向电泳免疫印迹法研究提取协议

Article DOI: 10.3791/51339-v • 10:51 min • April 10th, 2014
April 10th, 2014 •
1, 1,2, 1, 1, 1, 1, 1, 3, 4, 2, 3, 1, 1, 1, 1, 1
1Team Alzheimer & Tauopathies, Jean-Pierre Aubert Research Centre, Inserm UMR 837, 2EA 4308-Department of Reproductive Biology-Spermiology-CECOS, CHRU-Lille, 3EA2686-Laboratorie d'Immunologie, Faculté de Médecine - Pôle Recherche, 4Department of Neurology, CHRU-Lille

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用尿素/硫脲/ SDS缓冲液为人类和小鼠脑组织中一种常见的蛋白提取协议允许由蛋白质2D-DIGE并随后将其表征迷你2DE免疫印迹鉴定。这种方法使一个人从活组织切片检查和实验模型获得更多的可重复性和可靠的结果。

Tags

神经科学，第86期，蛋白质组学，神经变性，二维超声心动图，人类和小鼠脑组织，荧光，免疫印迹。
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