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Journal / Bioengineering / Lignin nedreglering av
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JoVE Journal Bioengineering
Lignin nedreglering av
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Lignin nedreglering av

Article DOI: 10.3791/51340-v • 14:43 min • July 23rd, 2014
July 23rd, 2014 •
1, 2, 3, 4
1The School of Plant Sciences, University of Arizona, 2Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, 3The Institute for Sustainable and Renewable Resources, The Institute for Advanced Learning and Research, 4Department of Plant, Soil and Microbial Sciences, Michigan State University

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En dubbelsträngad RNA-interferens (dsRNAi)-teknik används för att nedreglera cinnamoyl majs koenzym A-reduktas (ZmCCR1)-gen av lägre växtligninhalt. Lignin nedreglering från cellväggen visualiseras av mikroskopiska analyser och kvantifieras av Klason-metoden. Kompositionella förändringar av hemicellulosa och kristallin cellulosa analyseras.

Tags

Bioteknik , Cinnamoyl-CoA-reduktas (CCR) dsRNAi Klason lignin mätning cellväggen kolhydratanalys gaskromatografi (GC)
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