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Journal / Bioengineering / Lignin Aşağı düzenleme
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JoVE Journal Bioengineering
Lignin Aşağı düzenleme
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Lignin Aşağı düzenleme

Article DOI: 10.3791/51340-v • 14:43 min • July 23rd, 2014
July 23rd, 2014 •
1, 2, 3, 4
1The School of Plant Sciences, University of Arizona, 2Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, 3The Institute for Sustainable and Renewable Resources, The Institute for Advanced Learning and Research, 4Department of Plant, Soil and Microbial Sciences, Michigan State University

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Bir çift sarmallı RNA girişimi (dsRNAi) tekniği mısır sinamoil koenzim alt bitki lignin içeriğine A redüktaz (ZmCCR1) geni aşağı regüle etmek için kullanılır. Lignin hücre duvarından aşağı-regülasyon mikroskopik analiz ile görselleştirilmiştir ve Klason yöntemi ile ölçülür. Yarı selüloz ve kristalin selüloz içinde Bileşim değişiklikler analiz edilmiştir.

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Biyomühendislik Sayı 89, Sinamoil-CoA redüktaz (CCR) dsRNAi Klason lignin ölçümü hücre duvarı karbonhidrat analiz gaz kromatografisi (GC)
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