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Kvantificering af Breast Cancer Cell invasiv Ved hjælp af en tre-dimensionel (3D) Model
 
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Kvantificering af Breast Cancer Cell invasiv Ved hjælp af en tre-dimensionel (3D) Model

Article DOI: 10.3791/51341-v 08:08 min June 11th, 2014
June 11th, 2014

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Summary

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Click here for the English version.

Denne artikel indeholder detaljerede metoder til anvendelse af tre-dimensionelle (3D) assays at kvantificere brystkræft celle invasion. Konkret har vi diskutere de procedurer, der kræves for at oprette sådanne assays, kvantificering og dataanalyse, samt metoder til at undersøge tabet af membranintegritet der opstår, når celler invadere.

Tags

Medicine Brystkræft celle invasion ekstracellulær matrix (ECM) tre-dimensionelle (3D) kulturer immuncytokemi Matrigel basalmembranmatrix
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