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Kwantificering van de Breast Cancer Cell Invasiviteit met een drie-dimensionale (3D) Model
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Kwantificering van de Breast Cancer Cell Invasiviteit met een drie-dimensionale (3D) Model

Article DOI: 10.3791/51341-v • 08:08 min • June 11th, 2014
June 11th, 2014 •
*1, *1, 1,2,3
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, 2Department of Oncology, Schulich School of Medicine and Dentistry, University of Western Ontario, 3Lawson Health Research Institute
* These authors contributed equally

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Dit artikel geeft gedetailleerde methodologieën voor het gebruik van driedimensionale (3D) assays om borstkanker cel invasie kwantificeren. Concreet bespreken we het nodig om dergelijke assays procedures, kwantificering en gegevensanalyse, alsook werkwijzen voor het verlies van membraan integriteit die ontstaat wanneer cellen binnendringen onderzoeken.

Tags

Geneeskunde Borstkanker invasie extracellulaire matrix (ECM) driedimensionale (3D) culturen immunocytochemie Matrigel basaal membraan matrix
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