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Captura de glicopeptídeos de Superfície Celular Proteômica

Article DOI: 10.3791/51349-v • 10:11 min • May 9th, 2014
May 9th, 2014 •
1, 1
1Department of Chemistry, Simon Fraser University

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As proteínas de superfície são biologicamente importantes e amplamente glicosilada. Apresentamos aqui uma abordagem de captura de glycopeptide para solubilizar, enriquecer e deglycosylate estas proteínas para análise proteômica baseada LC-MS fáceis.

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Molecular Biology Edição 87 proteína de membrana uma glicoproteína ligada a N a modificação pós-translacional espectrometria de massa HPLC química hidrazida N-glycoproteomics captura glicopéptido
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