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May 19, 2014
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We describe the dissection and ex vivo culture of mouse embryonic skin. The culture system maintains an air-liquid interface across the tissue surface and allows imaging on an inverted microscope. Melanoblasts, a component of the developing skin, are fluorescently labeled allowing their behavior to be observed using confocal microscopy.
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Mort, R. L., Keighren, M., Hay, L., Jackson, I. J. Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration. J. Vis. Exp. (87), e51352, doi:10.3791/51352 (2014).
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