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Biology

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Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration
 

Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration

Article DOI: 10.3791/51352
May 19th, 2014

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Summary May 19th, 2014

We describe the dissection and ex vivo culture of mouse embryonic skin. The culture system maintains an air-liquid interface across the tissue surface and allows imaging on an inverted microscope. Melanoblasts, a component of the developing skin, are fluorescently labeled allowing their behavior to be observed using confocal microscopy.

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