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JoVE Journal Neuroscience
Usando um α-Bungarotoxina Binding Tag Site para Estudar GABA um receptor de membrana Localização e Tráfico
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Usando um α-Bungarotoxina Binding Tag Site para Estudar GABA um receptor de membrana Localização e Tráfico

Article DOI: 10.3791/51365
March 28th, 2014 •
1, 1, 1
1Pharmacology & Chemical Biology Department, University of Pittsburgh School of Medicine

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March 28th, 2014

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Aqui demonstramos a utilização do corante fluorescente Alexa acoplado a α-bungarotoxina para medir GABA A localização do receptor de superfície e endocitose em neurónios do hipocampo. Através da utilização de construções que ostentam uma marca curta extracelular que se liga α-bungarotoxina, análise de proteína da membrana plasmática do tráfico endocítica pode ser alcançado.

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