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Journal / Neuroscience / Laser Nanosurgery of Cerebellar Axons In Vivo
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JoVE Journal Neuroscience
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Laser Nanosurgery of Cerebellar Axons In Vivo

Article DOI: 10.3791/51371-v • 09:25 min • July 28th, 2014
July 28th, 2014 •
1, 1,2, 1,2,3,4
1European Laboratory for Non-Linear Spectroscopy, University of Florence, 2National Institute of Optics, National Research Council, 3Department of Physics and Astronomy, University of Florence, 4International Center for Computational Neurophotonics (ICON Foundation)

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Summary
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Two-photon imaging, coupled to laser nanodissection, are useful tools to study degenerative and regenerative processes in the central nervous system with subcellular resolution. This protocol shows how to label, image, and dissect single climbing fibers in the cerebellar cortex in vivo.

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Laser Nanosurgery Cerebellar Axons In Vivo Neuronal Regeneration CNS Adult Mammals Climbing Fibers Time-lapse Dynamics Two-photon Fluorescence Imaging Laser Surgery Selective Tool Fluorescent Structures Intact Mouse Cortex TPF Time-lapse Imaging Single Axonal Branches Cerebellum Olivocerebellar Neurons Anterograde Tracing Dextran-conjugated Dye Cranial Window Ti:Sapphire Laser Degeneration Regrowth
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