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Interferometria Bio-layer para Medição Cinética de interações proteína-proteína e alostéricas Ligante Effects
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Interferometria Bio-layer para Medição Cinética de interações proteína-proteína e alostéricas Ligante Effects

Article DOI: 10.3791/51383-v • 13:57 min • February 18th, 2014
February 18th, 2014 •
1, 1
1Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University

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Os protocolos aqui descrever os ensaios cinéticos de interações proteína-proteína com Bio-camada Interferometria. Tipo F ATP sintase, que está envolvida no metabolismo da energia celular, pode ser inibida pela sua subunidade ε em bactérias. Temos adaptado Bio-camada de interferometria para estudar as interacções do complexo catalítico com o domínio C-terminal do inibidor ε.

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Química ATP sintase Bio-Layer Interferometria mudança conformacional induzida pelo ligante Análise de Interação Biomolecular a regulação alostérica inibição de enzimas
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