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JoVE Journal Neuroscience
Super-Resolution Imaging neuronaler Dense-Core-Vesikel
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Super-Resolution Imaging neuronaler Dense-Core-Vesikel

Article DOI: 10.3791/51394-v • 09:30 min • July 2nd, 2014
July 2nd, 2014 •
1,2, 2, 3, 2,4
1Department of Physics, Lewis & Clark College, 2Program in Biochemistry and Molecular Biology, Lewis & Clark College, 3Jungers Center for Neuroscience Research, Oregon Health & Science University, 4Department of Chemistry, Lewis & Clark College

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Wir beschreiben, wie die photoaktivierte Lokalisationsmikroskopie (PALM)-basierte Studien von Vesikeln in festen, kultivierten Neuronen zu implementieren. Schlüsselkomponenten unseres Protokolls gehören Kennzeichnung Vesikel mit photoconvertible Chimären, sammeln spärlich abgetasteten RAW-Bilder mit einer superauflösende Mikroskopie-System, und die Verarbeitung der RAW-Bilder, eine Super-Resolution-Bild zu erzeugen.

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Neuroscience Ausgabe 89 photoaktivierte Lokalisationsmikroskopie Dendra2 Dense-Core-Vesikel Synapse Hippocampus Cluster-
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