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JoVE Journal Neuroscience
Immunohistochemische en Calcium Imaging Methods in Wholemount Rat Retina
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Immunohistochemische en Calcium Imaging Methods in Wholemount Rat Retina

Article DOI: 10.3791/51396-v • 08:54 min • October 13th, 2014
October 13th, 2014 •
1, 1,2,3, 1,2,4, 1
1Department of Neurobiology, University of California, Los Angeles, 2Veterans Administration Greater Los Angeles Healthcare System, 3Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences, Dalhousie University, 4Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine, University of California, Los Angeles

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Immunohistochemie protocollen worden gebruikt voor de lokalisatie van een specifiek eiwit in het netvlies te bestuderen. Calcium beeldvormende technieken worden gebruikt om calcium dynamiek in retinale ganglioncellen en hun axonen te bestuderen.

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Neurowetenschappen immunohistochemie antilichaam fluo-4 calcium imaging ganglioncellen netvlies rat
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