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JoVE Journal Neuroscience
Metodi di imaging immunoistochimici e di calcio in Wholemount Rat Retina
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Metodi di imaging immunoistochimici e di calcio in Wholemount Rat Retina

Article DOI: 10.3791/51396-v • 08:54 min • October 13th, 2014
October 13th, 2014 •
1, 1,2,3, 1,2,4, 1
1Department of Neurobiology, University of California, Los Angeles, 2Veterans Administration Greater Los Angeles Healthcare System, 3Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences, Dalhousie University, 4Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine, University of California, Los Angeles

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Immunoistochimica protocolli sono utilizzati per studiare la localizzazione di una specifica proteina nella retina. Tecniche di imaging di calcio sono impiegate per studiare le dinamiche del calcio nelle cellule gangliari retiniche e dei loro assoni.

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Neuroscienze immunoistochimica anticorpi fluo-4 l'imaging del calcio cellule gangliari retina ratto
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