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Journal / Immunology and Infection / İnsan nötrofil Akış odası Yapışma Deneyi
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
İnsan nötrofil Akış odası Yapışma Deneyi
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
Journal (Immunology and Infection)
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
Increased Recovery Time and Decreased LPS Administration to Study the Vagus Nerve Stimulation Mechanisms in Limited Inflammatory Responses
Journal (Immunology and Infection)
Increased Recovery Time and Decreased LPS Administration to Study the Vagus Nerve Stimulation Mechanisms in Limited Inflammatory Responses
 
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İnsan nötrofil Akış odası Yapışma Deneyi

Article DOI: 10.3791/51410 • 12:42 min • July 2nd, 2014
July 2nd, 2014 •
1, 2,3,4, 5, 5
1Genetics and Genomic Sciences Graduate Program, University of Alabama at Birmingham, 2Birmingham Veterans Affairs Medical Center, 3Department of Pathology, University of Alabama at Birmingham, 4Department of Biomedical Engineering, University of Alabama at Birmingham, 5Department of Medicine, University of Alabama at Birmingham

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Nötrofil yapışmasını ölçülmesi için bir yöntem rapor edilir. Bu yöntem, bir kan damarı karşılaşılan edilene benzer bir dinamik akış ortam oluşturur. Bu izin saflaştırılmış yapışma molekülleri (ligand) veya saf stres ile in vivo ortama benzer bir bağlamda endotel hücre alt-tabaka (HUVEC) ya da, nötrofil adhezyonunun araştırılması.

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Immunology Sayı 89 nötrofil yapışma akış odası insan göbek damarı endotel hücre (HUVEC) saflaştırılmış ligand
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