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Journal / Immunology and Infection / Humanen neutrophilen Flusskammer Adhesion Assay
JoVE Journal
Immunology and Infection

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Humanen neutrophilen Flusskammer Adhesion Assay
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Humanen neutrophilen Flusskammer Adhesion Assay

Article DOI: 10.3791/51410-v • 12:42 min • July 2nd, 2014
July 2nd, 2014 •
1, 2,3,4, 5, 5
1Genetics and Genomic Sciences Graduate Program, University of Alabama at Birmingham, 2Birmingham Veterans Affairs Medical Center, 3Department of Pathology, University of Alabama at Birmingham, 4Department of Biomedical Engineering, University of Alabama at Birmingham, 5Department of Medicine, University of Alabama at Birmingham

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Verfahren zum Quantifizieren von Adhäsion von Neutrophilen berichtet. Dieses Verfahren erzeugt eine dynamische Fließumgebung ähnlich der in einem Blutgefäß auftreten. Es erlaubt die Untersuchung der Adhäsion von Neutrophilen an entweder gereinigte Adhäsionsmoleküle (Ligand) oder Endothelzell-Substrat (HUVEC) in einem Rahmen ähnlich der in vivo Umgebung mit Scherbeanspruchung.

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Immunologie Neutrophilen-Adhäsion Flusskammer menschliche Nabelschnur Endothelzellen (HUVEC) gereinigt Liganden
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