Chapters
Summary
A protocol involving integrated concentration, enrichment, and end-point colorimetric detection of foodborne pathogens in large volumes of agricultural water is presented here. Water is filtered through Modified Moore Swabs (MMS), enriched with selective or non-selective media, and detection is performed using paper-based analytical devices (µPAD) imbedded with bacterial-indicative colorimetric substrates.
Tags
Colorimetric Paper-based Detection Escherichia Coli Salmonella Spp. Listeria Monocytogenes Agricultural Water Large Volumes Rapid Detection Modified Moore Swabs Gauze Filter Plastic Cartridge Bacteria Concentration Selective Enrichment Non-selective Enrichment Paper-based Analytical Devices (PADs) Bacteria-indicative Substrates Qualitative Detection Visual Detection Digital Images ImageJ Software Objective Interpretation Standardized Interpretation Biochemical Screening Procedures Bacterial Pathogens False Positive Confirmation Diagnostic
PLAYLIST
Antimicrobial Resistance
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Research • Immunology and InfectionVisualization of Bacterial Resistance using Fluorescent Antibiotic Probes -
Research • GeneticsQuantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach -
Research • Immunology and InfectionA Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment -
Research • BioengineeringBiosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria -
Research • EnvironmentScreening Foodstuffs for Class 1 Integrons and Gene Cassettes -
Research • BioengineeringHigh-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
Bacterial Pathogens
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Education • Core: BiologyBacterial Signaling -
Education • Lab BioMicrobial and Fungal Diversity- Concept -
Education • Environmental MicrobiologyIsolation of Fecal Bacteria from Water Samples by Filtration -
Education • Environmental MicrobiologyWater Quality Analysis via Indicator Organisms -
Education • MicrobiologyMicroscopy and Staining: Gram, Capsule, and Endospore Staining -
Research • Immunology and InfectionUse of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds -
Research • Immunology and InfectionLaboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Chemical Contaminants
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Research • EnvironmentLaboratory Estimation of Net Trophic Transfer Efficiencies of PCB Congeners to Lake Trout (Salvelinus namaycush) from Its Prey -
Research • BiochemistryStudy on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves -
Research • EnvironmentDetermination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry. -
Research • ChemistryPTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
Food Defense and Integrity
Food Preparation and Handling
Food Processing and Technology
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Education • Core: BiologyPlant Breeding and Biotechnology -
Education • Environmental SciencesTesting For Genetically Modified Foods -
Research • BiologyA Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling -
Research • Immunology and InfectionMonitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System -
Research • EnvironmentEcosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions -
Research • EnvironmentThe Effect of the Application of Thyme Essential Oil on Microbial Load During Meat Drying
Microbial Genetics
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Education • Microbiology16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species -
Research • BiologyGuided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing -
Education • Environmental MicrobiologyCommunity DNA Extraction from Bacterial Colonies -
Research • EnvironmentEnrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples -
Research • EnvironmentDetection of Foodborne Bacterial Pathogens from Individual Filth Flies -
Research • BioengineeringDevelopment of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen -
Research • EnvironmentTracking Microbial Contamination in Retail Environments Using Fluorescent Powder - A Retail Delicatessen Environment Example -
Research • Immunology and InfectionA High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
Microbiome
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Research • EnvironmentCharacterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities -
Research • Immunology and InfectionAn In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota -
Research • MedicineOral Biofilm Sampling for Microbiome Analysis in Healthy Children -
Research • GeneticsExploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere -
Research • EnvironmentA Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation
Natural Toxins
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Research • BiologyELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection -
Research • Immunology and InfectionQuantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays -
Research • ChemistryNatural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
Parasites
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Research • Immunology and InfectionOral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food -
Research • Immunology and InfectionDetermination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay -
Research • Immunology and InfectionA Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
Prevention and Control
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Research • BioengineeringThe Portable Chemical Sterilizer (PCS), D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military -
Research • EnvironmentColorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water -
Research • BiologyColorimetric Detection of Bacteria Using Litmus Test -
Research • Immunology and InfectionFoodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7 -
Research • Immunology and Infection'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens -
Research • Immunology and InfectionCombination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
Viruses and Prions
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Education • Core: BiologyRetrovirus Life Cycles -
Education • Core: BiologyViral Recombination -
Research • EnvironmentEPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples -
Research • EnvironmentEPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay -
Research • EnvironmentEPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR -
Research • Immunology and InfectionSwab Sampling Method for the Detection of Human Norovirus on Surfaces -
Research • Immunology and InfectionA Protocol for Analyzing Hepatitis C Virus Replication -
Research • Immunology and InfectionDevelopment of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle -
Research • Immunology and InfectionProcedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species
USDA Food Safety JoVE Publications
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Research • EnvironmentRNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem -
Research • BiologyA Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples -
Research • Immunology and InfectionGlass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens -
Research • EnvironmentInhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L. -
Research • EnvironmentControlled-release of Chlorine Dioxide in a Perforated Packaging System to Extend the Storage Life and Improve the Safety of Grape Tomatoes -
Research • EnvironmentProcedures of Laboratory Fumigation for Pest Control with Nitric Oxide Gas
