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JoVE Journal
Biology

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标记的细胞核从亲和力为基础的隔离
 
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标记的细胞核从亲和力为基础的隔离

Article DOI: 10.3791/51418-v 12:48 min March 25th, 2014
March 25th, 2014

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

果蝇组织中往往含有细胞类型的非均相混合物。为了检验从一个特定的组织特异性细胞类型的基因表达,细胞核可遗传标记,随后使用具有亲和力为基础的方法分离出来。分离的细胞核,可用于下游应用,如基因表达分析和染色质免疫沉淀。

Tags

生物化学,85期,基因表达,核隔离,
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