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JoVE Journal Neuroscience
Lineage-omprogrammering av Pericyte-deriverte celler av den voksne Human Brain inn Utførte Nerveceller
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Lineage-omprogrammering av Pericyte-deriverte celler av den voksne Human Brain inn Utførte Nerveceller

Article DOI: 10.3791/51433-v • 09:36 min • May 12th, 2014
May 12th, 2014 •
1, 2, 3, 1,4
1Department of Physiological Genomics, Institute of Physiology, Ludwig Maximilians University Munich, 2Tumor Biology Lab, Neurosurgical Clinic, Ludwig-Maximilians University Munich, 3Institut für Biochemie, Emil-Fischer-Zentrum, Friedrich-Alexander-Universität Erlangen-Nürnberg, 4Institute of Physiological Chemistry and Focus Program Translational Neuroscience, Johannes Gutenberg University Mainz

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Målretting hjerne-resident celler for direkte avstamning-omprogrammering gir nye perspektiver for hjernen reparasjon. Her beskriver vi en protokoll for hvordan du kan forberede kulturer beriket for hjerne-resident pericytes fra voksent menneske cerebral cortex og konvertere disse til induserte nevroner av retrovirus-mediert uttrykk for transkripsjonsfaktorene Sox2 og Ascl1.

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Neuroscience pericytes avstamning-omprogrammering indusert nevroner cerebral cortex
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