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JoVE Journal Neuroscience
Lineage-riprogrammazione di cellule pericyte-derivate del cervello umano adulto in neuroni indotti
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Lineage-riprogrammazione di cellule pericyte-derivate del cervello umano adulto in neuroni indotti

Article DOI: 10.3791/51433-v • 09:36 min • May 12th, 2014
May 12th, 2014 •
1, 2, 3, 1,4
1Department of Physiological Genomics, Institute of Physiology, Ludwig Maximilians University Munich, 2Tumor Biology Lab, Neurosurgical Clinic, Ludwig-Maximilians University Munich, 3Institut für Biochemie, Emil-Fischer-Zentrum, Friedrich-Alexander-Universität Erlangen-Nürnberg, 4Institute of Physiological Chemistry and Focus Program Translational Neuroscience, Johannes Gutenberg University Mainz

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Targeting cellule cerebrali residente per discendenza diretta-riprogrammazione offre nuove prospettive per la riparazione del cervello. Qui si descrive un protocollo di come preparare le culture arricchito da pericytes cervello residente da adulto corteccia cerebrale umana e convertirli in neuroni indotti dall'espressione retrovirus-mediata dei fattori di trascrizione Sox2 e Ascl1.

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Neuroscienze Numero 87 periciti lignaggio-riprogrammazione i neuroni indotti corteccia cerebrale
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