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Isolierung von Kleine-kodierenden RNAs von Human Serum
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Isolierung von Kleine-kodierenden RNAs von Human Serum

Article DOI: 10.3791/51443-v • 06:44 min • June 19th, 2014
June 19th, 2014 •
1, 1, 2,3
1School of Medical and Molecular Biosciences, Faculty of Science, University of Technology, Sydney, 2Centre for Health Technologies, Faculty of Engineering and Information Technology, University of Technology, Sydney, 3The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre, Royal Prince Alfred Hospital

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Dieses Protokoll beschreibt ein Verfahren zur Extraktion von kleinen RNAs aus menschlichem Serum. Wir haben diese Methode verwendet, um microRNAs von Krebsserum für den Einsatz in DNA-Arrays isolieren und auch Singleplex quantitative PCR. Das Protokoll nutzt Phenol und Guanidiniumthiocyanat Reagenzien mit Modifikationen, um qualitativ hochwertige RNA ergeben.

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Bioengineering kleine nicht-kodierende RNA-Isolierung microRNAs humanes Serum qPCR Guanidiniumthiocyanat Phase Lock Gels Arrays
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