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Insan serumundan küçük kodlayıcı olmayan RNA'lar izolasyonu
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Insan serumundan küçük kodlayıcı olmayan RNA'lar izolasyonu

Article DOI: 10.3791/51443-v • 06:44 min • June 19th, 2014
June 19th, 2014 •
1, 1, 2,3
1School of Medical and Molecular Biosciences, Faculty of Science, University of Technology, Sydney, 2Centre for Health Technologies, Faculty of Engineering and Information Technology, University of Technology, Sydney, 3The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre, Royal Prince Alfred Hospital

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Bu protokol, insan serumundan küçük RNA'ların ekstre edilmesi için bir yöntem tarif eder. Biz DNA dizileri kullanmak için kanser serumdan microRNA izole ve aynı zamanda singleplex kantitatif PCR için bu yöntemi kullandık. Protokol yüksek kalitede RNA elde edildi modifikasyonlarla fenol ve guanidinyum tiosiyanat reaktifler kullanır.

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Biyomühendislik Sayı 88 küçük noncoding RNA izolasyonu microRNA insan serum qPCR guanidiniyum tiyosiyanat Faz Kilit jeller diziler
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