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Combinando uma única molécula de Manipulação e imagem para o estudo das interações proteína-DNA
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Combinando uma única molécula de Manipulação e imagem para o estudo das interações proteína-DNA

Article DOI: 10.3791/51446-v • 14:43 min • August 27th, 2014
August 27th, 2014 •
1,2, 1, 1,3, 1,4,5,6, 1,4
1LENS - European Laboratory for Non-linear Spectroscopy, University of Florence, 2Chemistry Research Laboratory, University of Oxford, 3Department of Biology, University of Florence, 4Department of Physics and Astronomy, University of Florence, 5National Institute of Optics-National Research Council, Italy, 6International Center of Computational Neurophotonics

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Aqui nós descrevemos os instrumentos e métodos para a detecção de moléculas de proteína única fluorescently rotulados interagindo com uma única molécula de DNA suspenso entre duas microesferas opticamente presos.

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Bioengenharia biofísica das moléculas isoladas pinça óptica microscopia de fluorescência as proteínas se ligam ao DNA repressor lactose microfluídica
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