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JoVE Journal
Chemistry

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Hot Biological Catalysis
 

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Article DOI: 10.3791/51487-v 13:00 min April 4th, 2014
April 4th, 2014

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Summary

Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.

Tags

Isothermal Titration Calorimetry Enzymatic Reactions Thermodynamic Parameters Biomolecular Binding Equilibria Kinetics Kcat KM Enthalpy Canavalia Ensiformis Urease Urea Hydrolysis
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