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Hot Biologisk katalyse: Isothermal Titrering kalorimetri at karakterisere enzymatiske reaktioner

Article DOI: 10.3791/51487-v • 13:00 min • April 4th, 2014
April 4th, 2014 •
1, 1, 1
1Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna

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Isotermisk titrering kalorimetri foranstaltninger varmestrøm frigivet eller absorberet i kemiske reaktioner. Denne metode kan anvendes til at kvantificere enzym-katalyse. I dette papir, protokol for instrumental opsætning, eksperiment kører, og dataanalyse beskrives generelt, og anvendes til karakterisering af enzymatisk urea hydrolyse ved sværdbønne urease.

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Kemi Isotermisk titrering kalorimetri enzymatisk katalyse kinetik termodynamik entalpi Michaelis konstant katalytisk hastighedskonstant urease
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