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Hot Biological Katalyse
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Hot Biological Katalyse: Isotermisk Titrering Calorimetry å karakter enzymatiske reaksjoner

Article DOI: 10.3791/51487-v • 13:00 min • April 4th, 2014
April 4th, 2014 •
1, 1, 1
1Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna

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Isoterm titrering kalorimetri tiltak varmestrøm løslatt eller absorberes i kjemiske reaksjoner. Denne metoden kan brukes til å kvantifisere enzymkatalyse. I denne utredningen, protokollen for instrumental oppsett, eksperiment, og dataanalyse er generelt beskrevet, og brukes på karakterisering av enzymatisk urea hydrolyse av jack bønne urease.

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Kjemi Isoterm titrering kalorimetri enzymatisk katalyse kinetikk termodynamikk entalpi Michaelis konstant katalytisk hastighet konstant urease
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