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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Escalable de alto rendimiento seleccion de bibliotecas de anticuerpos sintéticos fagos
 
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Escalable de alto rendimiento seleccion de bibliotecas de anticuerpos sintéticos fagos

Article doi: 10.3791/51492
January 17th, 2015 •
1,2, 1,2, 1,2, 1,2, 1,3, 1,3, 1,4, 1,4, 1,3, 1,4, 1,4, 1,2
1The Recombinant Antibody Network, 2The Banting and Best Department of Medical Research, University of Toronto, 3Antibiome Center, University of California, San Francisco at Mission Bay, 4Department of Biochemistry and Molecular Biology, The University of Chicago

Summary
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January 17th, 2015

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Se describe un método con acompañamiento visual para conducir, las selecciones de rendimiento altos escalables de bibliotecas de anticuerpos sintéticos combinatorios fagos contra cientos de antígenos simultáneamente. El uso de este enfoque paralelo, hemos aislado fragmentos de anticuerpos que exhiben alta afinidad y especificidad para diversos antígenos que son funcionales en inmunoensayos estándar.

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