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Karakterisering af G-protein-koblede receptorer med en fluorescens-baserede calciummobilisering Assay

Article DOI: 10.3791/51516-v • 11:49 min • July 28th, 2014
July 28th, 2014 •
1, 1, 1, 1, 1, 1, 1
1Department of Biology, KU Leuven

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Den her beskrevne fluorescens-baserede calcium mobilisering analysen er en medium-throughput omvendt farmakologi screening til identifikation af funktionelt aktiverende ligand (r) af forældreløse G-protein-koblede receptorer (GPCR).

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Cellular Biology G-protein-koblet receptor (GPCR) calciummobilisering assay reverse farmakologi deorphanization cellulært ekspressionssystem HEK293T Fluo-4 FlexStation
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