Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Vaccinia Reporter Vira til kvantificering Viral…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Vaccinia Reporter Vira til kvantificering Viral Funktion på alle stadier af genekspression
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Journal (Immunology and Infection)
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Mycobacterial Antigen Preparation (Video) | JoVE
Journal (Immunology and Infection)
Mycobacterial Antigen Preparation (Video) | JoVE
 
Click here for the English version

Vaccinia Reporter Vira til kvantificering Viral Funktion på alle stadier af genekspression

Article DOI: 10.3791/51522-v • 10:48 min • May 15th, 2014
May 15th, 2014 •
1, 1, 1, 1
1Department of Microbiology, Boston University School of Medicine

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Vi beskriver brugen af ​​en fluorescerende reporter vacciniavirus, der muliggør real-time måling af virusinfektivitet og genekspression igennem etapen-specifikt udtryk for spektralt adskilte reporter fluorophores. Vi detalje en plade-baserede metode til præcist at identificere det stadium, hvor virus replikation påvirkes som reaktion på små molekyle hæmning.

Tags

Immunologi vaccinia; poxvirus; infektion; virus-vært-interaktion; skærmen; inhibitor; genekspression; cellebiologi; fluorescens; antiviral; reporter mCherry Venus TagBFP
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter