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Immunology and Infection

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JoVE Journal Immunology and Infection
Vaccinia Reporter Virus för kvantifiering Viral funktion i alla skeden av genuttryck
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Vaccinia Reporter Virus för kvantifiering Viral funktion i alla skeden av genuttryck

Article DOI: 10.3791/51522-v • 10:48 min • May 15th, 2014
May 15th, 2014 •
1, 1, 1, 1
1Department of Microbiology, Boston University School of Medicine

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Vi beskriver användningen av en fluorescerande reporter vacciniavirus som möjliggör realtidsmätning av viral smittsamhet och genuttryck genom scenen specifika uttryck för spektralt distinkta reporter fluoroforer. Vi detalj en plåt-baserad metod för exakt identifiering av det stadium då virusreplika påverkas som svar på små molekyler hämning.

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Immunologi vaccinia; poxvirus; infektion; virus-värd interaktioner; skärmen; hämmare; genexpression; cellbiologi; fluorescens; antiviralt; reporter mCherry Venus TagBFP
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