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对链球菌-Tactin树脂交联的双(磺基)辛二酸酯(BS3)双链球菌标记蛋白及其复合物的一步纯化
 
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对链球菌-Tactin树脂交联的双(磺基)辛二酸酯(BS3)双链球菌标记蛋白及其复合物的一步纯化

Article DOI: 10.3791/51536
April 20th, 2014

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Summary April 20th, 2014

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的方法被描述为在链亲和素修饰的双链球菌标记的融合蛋白和它们的特定配合物的净化效率(链球菌-Tactin)树脂共价交联的双(硫代琥珀酰亚胺基)辛二酸酯(BS3)。该方法具有速度快,良好的靶蛋白回收率和纯度高的优点,并且与随后的分析通过质谱兼容。

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