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Real-time beeldvorming van Single Engineered RNA-transcripten in levende cellen door gebruik Ratiometrische Bimoleculaire Beacons
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Real-time beeldvorming van Single Engineered RNA-transcripten in levende cellen door gebruik Ratiometrische Bimoleculaire Beacons

Article DOI: 10.3791/51544-v • 12:20 min • August 6th, 2014
August 6th, 2014 •
*1, *1, 2, 2, 1
1Department of Bioengineering, University of Pennsylvania, 2Integrated DNA Technologies, Inc.
* These authors contributed equally

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Ratiometrische bimoleculaire bakens (RBMBs) kan worden gebruikt om het interne gemanipuleerde RNA-transcripten in levende cellen. Hier beschrijven we de bereiding en zuivering van RBMBs, levering van RBMBs in cellen van microporatie en fluorescentie beeldvorming van afzonderlijke RNA transcripten in real-time.

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Genetica RNA imaging enkel molecuul fluorescentie levende cel
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