Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / siRNA Screening naar Ubiquitin en…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
siRNA Screening naar Ubiquitin en Ubiquitin-achtig systeem regulatoren van biologische pathways identificeren in gekweekte zoogdiercellen
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
Journal (Biology)
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
Author Spotlight: In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging (Video) | JoVE
Journal (Biology)
Author Spotlight: In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging (Video) | JoVE
 
Click here for the English version

siRNA Screening naar Ubiquitin en Ubiquitin-achtig systeem regulatoren van biologische pathways identificeren in gekweekte zoogdiercellen

Article DOI: 10.3791/51572-v • 10:43 min • May 24th, 2014
May 24th, 2014 •
1, 1, 1, 2, 1,2
1MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, 2Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Hier beschrijven we een methode om een ​​gerichte siRNA "ubiquitome" scherm om nieuwe ubiquitine en ubiquitine-achtige regulatoren van de-HIF1a gemedieerde cellulaire respons op hypoxie identificeren voeren. Dit kan worden aangepast aan elke biologische weg wanneer een robuuste uitlezing van reporter-activiteit beschikbaar.

Tags

Biochemie siRNA screening ubiquitine UBL ubiquitome hypoxie HIF1a High throughput zoogdiercellen luciferase reporter
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter