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JoVE Journal
Biology

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重组弹性蛋白样多肽及其融合物与从肽和蛋白质非色谱纯化
 
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重组弹性蛋白样多肽及其融合物与从肽和蛋白质非色谱纯化

Article DOI: 10.3791/51583-v 07:35 min June 9th, 2014
June 9th, 2014

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

弹性蛋白样多肽是刺激 - 反应的生物聚合物与应用,从重组蛋白纯化给药。这个协议描述了来自大肠杆菌的使用其低临界溶解温度的相变行为作为一个简单的替代色谱弹性蛋白样多肽和其肽或蛋白质融合的纯化和表征。

Tags

分子生物学,第88,弹性蛋白样多肽,低临界溶解温度,相分离,逆转变循环,蛋白质纯化,批次纯化
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