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Stimulering af Cytoplasmatiske DNA Sensing Pathways
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Stimulering af Cytoplasmatiske DNA Sensing Pathways

Article DOI: 10.3791/51593-v • 11:44 min • September 18th, 2014
September 18th, 2014 •
1, 1
1Department of Pathology, University of Cambridge

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Formålet med protokollen er at anvende optimale metoder til stimulering af de cytoplasmatiske DNA sensing veje i celler og in vivo. Dette opnås ved at forbedre dannelsen af ​​lange, dobbeltstrenget DNA under stumpendeligering. Celler eller mus transficeres derefter ved hjælp af et lipid transfektionsreagens.

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Cellebiologi medfødte immunitet DNA dobbeltstrenget DNA (dsDNA) concatemer signalering transfektion stimulering ligering
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