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Stimulering av cytoplasmatiske DNA Sensing Pathways
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Stimulering av cytoplasmatiske DNA Sensing Pathways

Article DOI: 10.3791/51593-v • 11:44 min • September 18th, 2014
September 18th, 2014 •
1, 1
1Department of Pathology, University of Cambridge

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Formålet med protokollen er å bruke optimale fremgangsmåter for å stimulere de cytoplasmiske DNA føler trasé i celler og in vivo. Dette oppnås ved å forbedre genereringen av lange, dobbeltkjedet DNA ved butt-ende ligering. Celler eller mus blir deretter transfektert ved hjelp av en lipid transfeksjon reagens.

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Cellular Biology medfødt immunitet DNA dobbelttrådet DNA (dsDNA) concatemer signalering transfeksjon stimulering ligation
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