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Immunology and Infection
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荧光目标阵列的T细胞反应评价中的应用<em>在体内</em
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Immunology and Infection
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JoVE Journal
Immunology and Infection
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses
In vivo
Please note that all translations are automatically generated.
Click here for the English version.
荧光目标阵列的T细胞反应评价中的应用<em>在体内</em
DOI:
10.3791/51627-v
•
12:09 min
•
June 19, 2014
•
Benjamin J. C. Quah
,
Danushka K. Wijesundara
,
Charani Ranasinghe
,
Christopher R. Parish
1
Department of Immunology, John Curtin School of Medical Research
,
Australian National University
Chapters
00:05
Title
01:39
Fluorescent Target Array Preparation: CTV and CFSE Labeling
04:54
FTA Preparation: Peptide Pulsing and Cell Washing
07:50
FTA Preparation: CPD Cell Labeling
09:43
FTA Assay in Response to VV/HIV Epitopes
11:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
监测
体内
详细的T细胞应答的能力,对于我们的免疫应答的理解的发展非常重要。在这里,我们描述了评估同时采用流式细胞仪> 250参数
体内
T细胞检测使用荧光目标阵列(自由贸易协定)的。
Tags
Fluorescent Target Array
FTA
CFSE
CTV
CPD
MHC Class I
MHC Class II
CD8+ T Cells
CD4+ T Cells
T Cell Response
In Vivo
Flow Cytometry
Antigen Epitopes
Recombinant Pox Virus Vaccine
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