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Journal / Biology / ADN-ARN combinado fluorescente
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ADN-ARN combinado fluorescente
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ADN-ARN combinado fluorescente

Article DOI: 10.3791/51628-v • 15:54 min • June 14th, 2014
June 14th, 2014 •
1, 1
1Department of Reproduction and Development, Erasmus MC - University Medical Center

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La hibridación fluorescente in situ (FISH) permite la detección de ácidos nucleicos en su ambiente nativo dentro de las células. Se describen un protocolo para el combinado, la detección simultánea de ARN y ADN por medio de pescado, que se puede utilizar para estudiar inactivación del cromosoma X en las células madre embrionarias de ratón.

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Bioquímica Fluorescente combinado de ADN-ARN FISH células ES citogenética el análisis de una sola célula inactivación del cromosoma X (XCI), Cromosoma artificial bacteriano (BAC) el ADN-sonda,
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