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ADN-ARN combiné Fluorescent

Article DOI: 10.3791/51628-v • 15:54 min • June 14th, 2014
June 14th, 2014 •
1, 1
1Department of Reproduction and Development, Erasmus MC - University Medical Center

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Hybridation in situ fluorescente (FISH) permet la détection d'acides nucléiques de leur environnement natif dans les cellules. Nous décrivons ici un protocole pour le combiné, la détection simultanée de l'ARN et de l'ADN à l'aide de FISH, qui peut être utilisée pour étudier l'inactivation du chromosome X dans les cellules souches embryonnaires de souris.

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Biochimie Numéro 88 Fluorescent ADN-ARN combinée FISH cellules ES cytogénétique analyse d'une seule cellule inactivation du chromosome X (XCI), Bactérienne chromosome artificiel (BAC) ADN-sonde,
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