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Journal / Biology / Un canale Cell-allegato patch-clamp registrazione
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Un canale Cell-allegato patch-clamp registrazione

Article DOI: 10.3791/51629-v • 13:07 min • June 9th, 2014
June 9th, 2014 •
1, 2, 1, 3, 4
1Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY, 2Department of Biochemistry, SMBS, University at Buffalo, SUNY, 3Molecular and Cellular Neuroscience Department, The Scripps Research Institute, 4Department of Biochemistry and Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY

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Qui viene descritta una procedura per ottenere lunghi tratti di registrazione corrente da un canale ionico con la tecnica patch-clamp cell-attached. Questo metodo permette di osservare, in tempo reale, il modello di apertura-chiusura conformazioni di canale che stanno alla base del segnale biologico. Questi dati informano sulle proprietà dei canali nelle membrane biologiche inalterate.

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Neuroscienze Numero 88 biofisica canali ionici la registrazione monocanale recettori NMDA gating elettrofisiologia patch-clamp analisi cinetica
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