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Identificatie van eiwit interacties Partners in zoogdiercellen behulp SILAC-immunoprecipitaties Kwantitatieve Proteomics
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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Journal (Biology)
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
 
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Identificatie van eiwit interacties Partners in zoogdiercellen behulp SILAC-immunoprecipitaties Kwantitatieve Proteomics

Article DOI: 10.3791/51656-v • 12:53 min • July 6th, 2014
July 6th, 2014 •
1, 1
1Division of Virology, Department of Pathology, University of Cambridge

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SILAC immunoprecipitatie experimenten vormen een krachtig middel voor het ontdekken van nieuwe eiwit: eiwit interacties. Doordat de nauwkeurige relatieve kwantificering van eiwitgehalte in zowel controle monsters, waar interacties gemakkelijk kan worden onderscheiden van experimenteel verontreinigingen en lage affiniteit interacties bewaard door het gebruik van minder stringente buffer omstandigheden.

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Biochemie massaspectrometrie weefselkweek technieken isotooplabelen SILAC stabiele isotopen Labeling van aminozuren in Cell Culture proteomics Interactomics immunoprecipitatie pulldown eIF4A GFP nanotrap orbitrap
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