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July 06, 2014
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Esperimenti di immunoprecipitazione SILAC rappresentano un potente mezzo per scoprire nuova proteina: interazioni proteina. Consentendo l'accurata quantificazione relativa abbondanza di proteine sia nel controllo e campioni di prova, veri interazione può essere facilmente distinto da contaminanti sperimentali e interazioni bassa affinità conservata attraverso l'uso di condizioni di buffer meno stringenti.
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Cite this Article
Emmott, E., Goodfellow, I. Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics. J. Vis. Exp. (89), e51656, doi:10.3791/51656 (2014).
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