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Identifisering av protein interaksjon Partnere i pattedyrceller Bruke SILAC-immunoprecipitation Kvantitative Proteomikk
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Identifisering av protein interaksjon Partnere i pattedyrceller Bruke SILAC-immunoprecipitation Kvantitative Proteomikk

Article DOI: 10.3791/51656-v • 12:53 min • July 6th, 2014
July 6th, 2014 •
1, 1
1Division of Virology, Department of Pathology, University of Cambridge

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SILAC immunoprecipitation eksperimenter representerer et kraftig middel for å oppdage romanen protein: protein interaksjoner. Ved å la den nøyaktige relative kvantifisering av protein overflod i både kontroll-og testprøver, sanne interaksjoner kan lett skilles fra eksperimentelle forurensninger, og en lav affinitet interaksjoner konservert ved bruk av mindre strenge bufferbetingelser.

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Biokjemi massespektrometri vev kultur teknikker isotop merking SILAC stabile isotoper Merking av aminosyrer i cellekultur proteomikk Interactomics immunoprecipitation nedtrekk eIF4A GFP nanotrap Orbitrap
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