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JoVE Journal Bioengineering
Glutamine Flux Imaging Using Genetically Encoded Sensors
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Glutamine Flux Imaging Using Genetically Encoded Sensors

Article DOI: 10.3791/51657-v • 10:23 min • July 31st, 2014
July 31st, 2014 •
1, 1
1Virginia Tech

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This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine sensors.

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Glutamine Flux Imaging Genetically Encoded Sensors Real-time Monitoring Subcellular Resolution Biological Molecules Transport Processes Kinetics Substrate Specificities Cellular Level Cell-type Specific Analyses Transporter Dynamics Glutamine Sensor Experimental Design Technical Details Post-experimental Analyses
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