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Journal / Biology / Studere DNA Looping af Single-Molecule FRET
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Studere DNA Looping af Single-Molecule FRET
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Studere DNA Looping af Single-Molecule FRET

Article DOI: 10.3791/51667-v • 11:27 min • June 28th, 2014
June 28th, 2014 •
1, 1
1School of Physics, Georgia Institute of Technology

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Denne undersøgelse viser en detaljeret eksperimentel fremgangsmåde til at måle looping dynamik dobbeltstrenget DNA under anvendelse af enkelt-molekyle Fluorescence Resonance Energy Transfer (FRET). Protokollen beskriver også, hvordan at udtrække looping sandsynlighedsfordelingen kaldet J faktor.

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Molekylær Biologi DNA looping J-faktor enkelt molekyle ærgre gelmobilitetsændring DNA krumning Worm-lignende kæde
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