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Het bestuderen van DNA Looping door Single-Molecule FRET
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Het bestuderen van DNA Looping door Single-Molecule FRET

Article DOI: 10.3791/51667-v • 11:27 min • June 28th, 2014
June 28th, 2014 •
1, 1
1School of Physics, Georgia Institute of Technology

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Deze studie geeft een gedetailleerde experimentele procedure om looping dynamiek van dubbelstrengs DNA meten met behulp van single-molecule Fluorescence Resonance Energy Transfer (FRET). Het protocol beschrijft ook hoe de looping kansdichtheid genaamd de J-factor halen.

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Molecular Biology DNA looping J-factor Single molecule FRET Gel mobiliteit shift DNA kromming worm-achtige keten
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