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Studera DNA Looping med enda molekyl FRET
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Studera DNA Looping med enda molekyl FRET

Article DOI: 10.3791/51667-v • 11:27 min • June 28th, 2014
June 28th, 2014 •
1, 1
1School of Physics, Georgia Institute of Technology

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Denna studie visar en detaljerad experimentell procedur för att mäta looping dynamik dubbelsträngat DNA med användning av en enda molekyl fluorescensresonansenergiöverföring (FRET). Protokollet beskriver också hur man extrahera looping sannolikhetstätheten kallas J faktorn.

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Molecular Biology DNA-looping J faktor enda molekyl FRET gel rörlighet skift DNA krökning maskliknande kedja
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