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JoVE Journal
Biology

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造血干细胞和祖细胞利用共聚焦和多光子显微镜五荧光蛋白标记的活体克隆跟踪
 
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造血干细胞和祖细胞利用共聚焦和多光子显微镜五荧光蛋白标记的活体克隆跟踪

Article DOI: 10.3791/51669-v 17:08 min August 6th, 2014
August 6th, 2014

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Summary

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Click here for the English version.

组合5的荧光蛋白的造血干细胞和祖细胞的标记允许通过共聚焦和双光子显微镜体内克隆跟踪,再生期间提供见解骨髓造血体系结构。此方法允许频谱编码HSPCs来源的细胞在完整组织的时间移植后广泛周期的非侵入性的命运映射。

Tags

干细胞生物学,第90,LEGO成像,克隆跟踪,荧光蛋白,共聚焦显微镜,多光子显微镜,造血,慢病毒载体的造血干细胞
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